We’re excited to announce that in 2024, we’ll be introducing an expansion to our lineup of PNA monomers. We’ve been diligently working on this project for a while, and we’re eager to unveil it. Keep reading to discover more about the new offerings we’ll be providing.
- Mmt-PNA-OH and Dmt-PNA-OH
PNAs are synthesized using SPS by anchoring the C-terminal monomer to a linker through its carboxylic acid on a solid support. After performing the elongation, the oligomer needs to be cleaved from the solid support. To do that, rather harsh reaction conditions are required, especially when standard Fmoc/Bhoc or Boc/Z monomers are utilized in the synthesis. These harsh cleaving conditions are incompatible with the synthesis of PNA/DNA chimeras, which have new properties, such as improved aqueous solubility and better cellular uptake. Most interestingly, PNA/DNA chimeras are recognized as substrates by various nucleic acid processing enzymes, and consequently can also assume biological functions, such as a primer function for DNA polymerases. The use of Mmt- or Dmt-protected PNAs enables the synthesis of chimeras by applying mild cleaving conditions.
Gamma-serine PNA monomers, which have a modified chemical structure, have been shown to have improved solubility and therefore tend less to precipitate from solution. With the introduction of Fmoc-protected gamma-Serine PNAs, we provide monomers that are compatible with automated solid-phase synthesis (SPS).
The advantage of Boc over Bhoc as the PG on the nucleobase is that Boc is a so-called traceless protecting group, meaning that the decomposition products after deprotection are gas (CO2) and solvent (t-BuOH) and can be removed quite easily. This traceless removal results in higher purity of the oligomer, leading to easier chromatographic purification of the final product.
- Fmoc-B+-PNA-OH (cationic monomers)
Peptide nucleic acids (PNAs) are antisense molecules with excellent polynucleotide hybridization properties; they are resistant to nuclease degradation but often have poor cell permeability leading to moderate cellular activity and limited clinical results. The addition of cationic substitutions (positive charges) to PNA molecules greatly increases cell permeability and cell-based activity without the use of permeability agents such as transfection reagents or phospholipids.
- PNA Intermediates (nucleic acids)
This is an addition to the already offered backbones.